Assets disappear when preview is launched

When I put an asset on a different layer other than the base layer the (newly) added asset disappears when I launch the game, also premade starters are not affected by this, it’s only when I add something new to said layer. Please help me (I accidentally deleted the UI while recording the video XD)
Screen-recording-2023-05-25-9015

Note: I had to compress and turn this into a gif so the video is a bit funky