Question about Admob Interstitial

Hello there.

I have Admob on my game. I have set the events such that:

At the beginning of the scene → Load interstitial


in collision with home (or level complete) → Show interstitial

The scenario is that the advertisement will be shown at the completion of level. If the player does not complete the level or loses a life and the level restarts, will the system load another advertisement, or a new advertisement will not load until the previously loaded advertisement is not shown? What will happen?